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KMID : 0861020110260040039
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Korea Journal of Herbology
2011 Volume.26 No. 4 p.39 ~ p.47
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Antioxidative and Anti-inflammatory Effects of Extracts from Different Organs of Cirsium japonicum var. ussuriense
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Mok Ji-Ye
Kang Hyun-Ju
Cho Jung-Keun
Jeon In-Hwa
Kim Hyeon-Soo
Park Ji-Min
Jeong Seung-Il
Shim J.S.
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Abstract
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Objective: The roots, leaves, flowers, stems and seeds of Cirsium japonicum var. ussuriense are often used in
treatment of human diseases such as hemorrhage, blood congestion and inflammation. Focusing our attention
on natural and bioavailable sources of antioxidants and anti-inflammation, we undertook to investigate the
antioxidant and anti-inflammatory properties of Cirsium japonicum var. ussuriense used as a folk medicine in
Korea.
Methods: The extracts of the leaves, stems, flowers, seeds and roots from C. japonicum var. ussuriense were
prepared by extracting with water or 80% ethanol. Total flavonoids and polyphenols were measured by a
colorimetric assay. The free radical scavenging activity of the extract was analyzed by the DPPH
(1,1-diphenyl-2-picryl hydrazyl), ABTS (2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and Griess
reagent assay. An oxidative product of nitric oxide (NO), was measured in the culture medium by the Griess
reaction. The level of prostaglandin E2 (PGE2) was measured by enzyme-linked immunosorbent assay. The
expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were measured by Western
blot analysis.
Results: Total flavonoid and polyphenol amounts of the leaves (CLE) and flowers (CFE) showed higher than
those of the seed extract (CSE), stem extract (CSTE) and roots (CRE). CLE and CFE also showed the high
antioxidant activities such as DPPH, NO-like and ABTS radical scavenging activity. An antioxidant activities of
these water extracts showed higher than those of 80% ethanol extracts. We investigated the anti-inflammatory
effects of CLE on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. CLE significantly suppressed the levels
of the inflammatory mediators such as NO and prostaglandin E2 (PGE2) in dose dependant. Furthermore, the
levels of iNOS and COX-2 protein expressions were markedly suppressed by the treatment with CLE extract in
a dose dependent manner.
Conclusions: These results suggest that CLE water extract has a higher anoxidant and anti-inflammatory
activity, these properties may contribute to the oxidative and inflammatory related disease care.
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KEYWORD
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Cirsium japonicum var. ussuriense, Water extract, antioxidant, anti-inflammation
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